The Global Penicillin G Acylase Market Size was worth around USD 148.30 Million in 2024, growing to USD 156.54 Million in 2025, and is predicted to grow to around USD 263.82 Million by 2035 with a compound annual growth rate (CAGR) of 5.38% from 2025 to 2035. The expansion of the penicillin G acylase market is influenced by growing demand for semi-synthetic antibiotics, rising antibiotic resistance, and increasing pharma production. Advances in enzyme manufacture, the availability of economical biocatalysis procedures, and the need for more healthcare in developing economies additionally propel market growth, and enzyme-based synthesis becomes increasingly appealing.